Chromatograms Subtraction

This feature is intended to subtract the background of your datasets or to apply a chromatogram or UV subtraction. See also these scripts.

First of all, you will need to load your dataset, which will display the main functions in the MS browser. Next click on the 'Add MS Dataset' button to select the file of your blank function (if the dataset contains the foreground and blank functions, you can skip this step):

Next, select the main function and click on the 'MS Subtraction' button to select the function to be subtracted (blank):

Click on OK to get the result. If you are interested in applying any normalization, feel free to check the applicable box (and enter the desired value) before having clicked on the OK button.

The MS Browser will display a line under the 'Subtraction' column, to display that a subtraction was applied:

You can remove the subtraction, just by selecting the function and clicking again on the MS subtraction button.

In the case that you are working with UV chromatograms, the workflow will be the same. In this case, you will need to select the DAD (or PDA) as the main function and subtract the applicable blank:

After having clicked on the OK button, you will get the expected result, with the applicable information under the 'Subtraction' column:

You can also subtract both TIC and DAD, just by selecting both subtractions: